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Multisensory Stripping Voltammetry Method for Ophthalmic Identification

Authors: Kolesnichenko I.I., Doronin A.N., Kantarzhi E.P. Published: 13.04.2018
Published in issue: #2(77)/2018  
DOI: 10.18698/1812-3368-2018-2-107-113

 
Category: Chemistry | Chapter: Electrochemistry  
Keywords: multisensory stripping voltammetry, рlanar electrodes, betaxolol, tear fluid

The paper addresses the suitability of the new electrochemical analytical method of multisensory stripping voltammetry for determining ophthalmic betaxolol. This method is used to identify organic substances that form complexes with metal cations in solution. As a result of this reaction, electroactivity of metal cations changes; these changes are detected by the stripping voltammetry method. We propose a test system that consists of cations of Zn2+, Cd2+, Рb2+, Со2+, Hg2+ in 0.05 М KCl solutions. When an analyze sample is injected into the test system, the metal cations interact with organic compounds; a stripping voltammogram reflects their effect on metal dissolution. So, organic substances are determined. Findings of the research show that betaxolol forms complex compounds with metal cations in the test system and in tear fluid, thereby affecting their electrochemical activity. Results suggest that the amount of electrodeposited metal is dependent on the concentration of betaxоlol, which made it possible to identify betaxоlol. We studied how after instillation of betaxolol tear fluid affects the test system for both healthy people and glaucoma patients. We found that betaxolol in the tear fluid of glaucoma patients promotes the formation of complexes with metal cations of the test-system, as compared to healthy people. The concentration of betaxolol in the tear fluid of glaucoma patients remains constant for 12 hours

References

[1] Andreev V.N., Ganshin V.M., Doronin A.N., et al. Sposob elektrokhimicheskogo multisensornogo obnaruzheniya i identifikatsii alkaloidov [Electrochemical multisensory method of alkaloids detecting and identification]. Patent 2375705 RF. Appl. 22.08.2008, publ. 10.12.2009 (in Russ.).

[2] Lukovtsev V.P., Doronin A.N., Lukovtseva N.V., Semenova V.A., Ganshin V.M. Identification of alkaloids using the stripping voltammetry method. Russian Journal of Electrochemistry, 2009, vol. 45, iss. 7, pp. 810–812. DOI: 10.1134/S1023193509070167

[3] Kolesnichenko I.I., Ganshin V.M., Doronin A.N., Lukovtsev V.P. Determination of antioxidants using multisensor stripping voltammetry. Sb. nauch. trudov mezhdunar. nauch. konf. «Izmeritelnye i informatsionnye tekhnologii v okhrane zdorovya» [Proc. Int. Sc. Conf. Measurement and information technologies in health protection]. Saint Petersburg, 2011, pp. 164–173 (in Russ.).

[4] Kolesnichenko I.I., Ganshin V.M., Klyuev A.L., et al. Possibility research of using multisensory stripping voltammetry for the analysis of volatile components of blood serum for medical diagnosis. Nauch. trudy 4-y mezhdunar. nauch.-prakt. konf. «Izmereniya v sovremennom mire — 2013» [Proc. 4th Int. Sc.-Practice Conf. Measurements Modern World — 2013]. Saint Petersburg, SPBU Publ., 2013, pp. 189–198 (in Russ.).

[5] Kolisnichenko I.I., Klyuev A.L., Ganshin V.M., Kantarji E.P., Doronin A.N., Lukovtsev V.P., Andreev V.N., Yemets V.V., Semyonova V.A. Express screening of biological objects using multisensory inversion voltammetry with pattern recognition. Protection of Metals and Physical Chemistry of Surfaces, 2014, vol. 50, iss. 4, pp. 543–547. DOI: 10.1134/S2070205114040078

[6] Kolesnichenko I.I., Doronin A.A., Lukovtsev V.P., Kantarzhi E.P. Express screening of biological objects state depending on the time of their storage. Sb. nauch. trudov 5-y Vserossiyskoy nauch.-prakt. konf. «Izmereniya v sovremennom mire — 2015» [Proc. 5th Russian Sc.-Pract. Conf. Measurements in Modern World — 2015]. Saint Petersburg, SPBU GPU Publ., 2015, pp. 141–146 (in Russ.).

[7] Kolesnichenko I.I., Balashova L.M., Kantarzhi E.P. Express screening of biological objects using multisensor stripping voltamperometry with pattern recognition. AJAC, 2016, vol. 7, no. 7, pp. 588–596. DOI: 10.4236/ajac.2016.77054

[8] Kolesnichenko I.I., Kantarzhi E.P., Doronin A.N. Using the method of multi-touch stripping voltammetry for the determination of visomitin in the tear fluid. Sb. nauch. trudov 6-y Vserossiyskoy nauch.-prakt. konf. «Izmereniya v sovremennom mire — 2017» [Proc. 6th Russ. Sc.-Pract. Conf. Measurements in Modern World — 2017]. Saint Petersburg, SPb GPU Publ., 2017, pp. 33–37 (in Russ.).

[9] Balashova L.M., Kolesnichenko I.I., Kantarzhi Ye.P. Determination of visomitin using the multisensory stripping voltammetry. Klinicheskaya gerontologiya [Clinical Gerontology], 2017, vol. 23, no. 9-10, pp. 6–8 (in Russ.).